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'''Non-photochemical quenching''' (NPQ) is a mechanism employed by plants and algae to protect themselves from the adverse effects of high light intensity.<ref name=Horton>{{cite journal | doi = 10.1093/jxb/eri023 | title = Regulation of Photosynthesis under Stress: Molecular design of the photosystem II light-harvesting antenna: photosynthesis and photoprotection | pmid = 15557295 | journal = J. Exp. Bot. | date = 2005-04 | first = Peter | last = Horton | coauthors = Alexander V. Ruban | volume = 56 | issue = 411 | pages = 365–373 | url = http://jxb.oxfordjournals.org/content/56/411/365.abstract?sid=9489ebee-5b2f-4292-9260-54cea443c6b8 | accessdate = 2011-01-15}}</ref>  It involves the quenching of singlet [[excited state]] [[chlorophyll]]s (Chl) via enhanced [[internal conversion (chemistry)|internal conversion]] to the [[ground state]] (non-radiative decay), thus harmlessly dissipating excess excitation energy as heat through molecular vibrations. NPQ occurs in almost all photosynthetic [[eukaryote]]s (algae and plants), and helps to regulate and protect photosynthesis in environments where [[light energy]] [[Absorption (electromagnetic radiation)|absorption]] exceeds the capacity for light utilization in [[photosynthesis]].<ref name=muller>{{cite journal | doi = 10.1104/pp.125.4.1558 | title = Update on Photosynthesis: Non-Photochemical Quenching. A Response to Excess Light Energy | journal = Plant Physiol | date = 2001-04 | first = Patricia | last = Müller | pmid = 11299337 | coauthors = Xiao-Ping Li, Krishna K. Niyogi | volume = 125 | issue = 4 | pmc = 1539381 | pages = 1558–1566 | id = | url = http://www.plantphysiol.org/cgi/content/full/125/4/1558 | accessdate = 2010-08-04}}</ref>


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==Process==
[[File:Photosynthetic parameters of plants.png|thumb|Carbon assimilation (red line) tends to saturate at high light intensities, while light absorption (blue line) increases linearly<ref name="tamoi">{{cite journal|author=Masahiro Tamoi, Miki Nagaoka, Yoshiko Miyagawa and Shigeru Shigeoka|year=2006|title=Contribution of Fructose-1,6-bisphosphatase and Sedoheptulose-1,7-bisphosphatase to the Photosynthetic Rate and Carbon Flow in the Calvin Cycle in Transgenic Plants|journal=Plant & Cell Physiology|volume=29|issue=10|pages=380&ndash;390|publisher=Japanese Society of Plant Physiologists|doi=10.1093/pcp/pcj004|issn=1471-9053|url=http://pcp.oxfordjournals.org/content/47/3/380/F4.expansion.html|accessdate= }}</ref>]]
[[File:Irradiance and carbon assimilation for a monoculture of Woloszynskia halophila at different pH.png|thumb|The relationship between irradiance and carbon assimilation for a [[monoculture]] of the [[plankton]] ''[[Woloszynskia halophila]]'' at different [[pH]]<ref name="spilling">{{cite journal|author=Kristian Spilling|year=2007|title=Dense sub-ice bloom of dinoflagellates in the Baltic Sea, potentially limited by high pH|journal=Journal of Plankton Research|volume=29|issue=10|pages=895&ndash;901|publisher=Oxford University Press|doi=10.1093/plankt/fbm067|issn=1464-3774|url=http://plankt.oxfordjournals.org/content/29/10/895/F4.expansion.html|accessdate= }}</ref>]]
When a molecule of [[chlorophyll]] absorbs light it is promoted from its [[ground state]] to its first singlet excited state. The excited state then has three main fates. Either the energy is; 1. passed to another chlorophyll molecule by [[Förster resonance energy transfer]] (in this way excitation is gradually passed to the photochemical reaction centers ([[photosystem I]] and [[photosystem II]]) where energy is used in photosynthesis (called photochemical quenching); or 2. the excited state can return to the ground state by emitting the energy as heat (called non-photochemical quenching); or 3. the excited state can return to the ground state by emitting a photon ([[fluorescence]]).  
 
In higher plants, the absorption of light continues to increase as light intensity increases, while the capacity for photosynthesis tends to saturate. Therefore there is the potential for the absorption of excess light energy by photosynthetic [[Photosynthesis#Photosynthetic membranes and organelles|light harvesting]] systems. This excess excitation energy leads to an increase in the lifetime of singlet excited [[chlorophyll]], increasing the chances of the formation of long-lived chlorophyll triplet states by [[inter-system crossing]]. Triplet chlorophyll is a potent [[photosensitiser]] of molecular oxygen forming [[singlet oxygen]] which can cause oxidative damage to the pigments, lipids and proteins of the photosynthetic [[thylakoid membrane]]. To counter this problem, one photoprotective mechanism is so-called non-photochemical quenching (NPQ), which relies upon the conversion and dissipation of the excess excitation energy into heat. NPQ involves conformational changes within the light harvesting proteins of photosystem II that bring about a change in pigment interactions causing the formation of energy traps. The conformational changes are stimulated by a combination of transmembrane proton gradient, the PsbS subunit of [[photosystem II]] and the enzymatic conversion of the carotenoid [[violaxanthin]] to [[zeaxanthin]] (the [[xanthophyll cycle]]).
 
''For further discussion, see [[Chlorophyll fluorescence]] and [[Plant stress measurement]].''
 
==Measurement of NPQ==
Non-photochemical quenching is measured by the quenching of chlorophyll fluorescence and is distinguished from photochemical quenching by applying a bright light pulse to transiently saturate photochemical quenching thus removing its contribution from the observed quenching. Non-photochemical quenching is not affected if the pulse of light is short. During the pulse, the fluorescence reaches the level reached in the absence of any photochemical quenching, known as maximum fluorescence, <math>F_m</math>.
 
''For further discussion, see [[Chlorophyll fluorescence#Measuring fluorescence|Measuring chlorophyll fluorescence]] and [[Plant stress measurement]].''
 
Chlorophyll fluorescence can easily be measured with a chlorophyll fluorometer. Some fluorometers (such as the [http://www.adc.co.uk/Products/NEW_ADC_OS5p_Advanced_Modulated_Fluorometer/ OS-5p]) can calculate NPQ and photochemical quenching coefficients (including qP, qN, qE and NPQ), as well as light and dark adaptation parameters (including Fo, Fm, and Fv/Fm).
 
== See also ==
[[Chlorophyll fluorescence]]
 
[[Chlorophyll fluorescence#Measuring fluorescence|Measuring chlorophyll fluorescence]]
 
==References==
 
{{Reflist}}
 
          5.   Jörg Standfuss, Anke C Terwisscha van Scheltinga, Matteo Lamborghini and Werner Kühlbrandt, Mechanisms of photoprotection and nonphotochemical quenching in pea lightharvesting complex <br />
                at 2.5A° resolution The EMBO Journal VOL 24 | NO 5 | 200
 
[[Category:Photosynthesis]]

Revision as of 15:45, 28 January 2014

Non-photochemical quenching (NPQ) is a mechanism employed by plants and algae to protect themselves from the adverse effects of high light intensity.[1] It involves the quenching of singlet excited state chlorophylls (Chl) via enhanced internal conversion to the ground state (non-radiative decay), thus harmlessly dissipating excess excitation energy as heat through molecular vibrations. NPQ occurs in almost all photosynthetic eukaryotes (algae and plants), and helps to regulate and protect photosynthesis in environments where light energy absorption exceeds the capacity for light utilization in photosynthesis.[2]

Process

Carbon assimilation (red line) tends to saturate at high light intensities, while light absorption (blue line) increases linearly[3]
The relationship between irradiance and carbon assimilation for a monoculture of the plankton Woloszynskia halophila at different pH[4]

When a molecule of chlorophyll absorbs light it is promoted from its ground state to its first singlet excited state. The excited state then has three main fates. Either the energy is; 1. passed to another chlorophyll molecule by Förster resonance energy transfer (in this way excitation is gradually passed to the photochemical reaction centers (photosystem I and photosystem II) where energy is used in photosynthesis (called photochemical quenching); or 2. the excited state can return to the ground state by emitting the energy as heat (called non-photochemical quenching); or 3. the excited state can return to the ground state by emitting a photon (fluorescence).

In higher plants, the absorption of light continues to increase as light intensity increases, while the capacity for photosynthesis tends to saturate. Therefore there is the potential for the absorption of excess light energy by photosynthetic light harvesting systems. This excess excitation energy leads to an increase in the lifetime of singlet excited chlorophyll, increasing the chances of the formation of long-lived chlorophyll triplet states by inter-system crossing. Triplet chlorophyll is a potent photosensitiser of molecular oxygen forming singlet oxygen which can cause oxidative damage to the pigments, lipids and proteins of the photosynthetic thylakoid membrane. To counter this problem, one photoprotective mechanism is so-called non-photochemical quenching (NPQ), which relies upon the conversion and dissipation of the excess excitation energy into heat. NPQ involves conformational changes within the light harvesting proteins of photosystem II that bring about a change in pigment interactions causing the formation of energy traps. The conformational changes are stimulated by a combination of transmembrane proton gradient, the PsbS subunit of photosystem II and the enzymatic conversion of the carotenoid violaxanthin to zeaxanthin (the xanthophyll cycle).

For further discussion, see Chlorophyll fluorescence and Plant stress measurement.

Measurement of NPQ

Non-photochemical quenching is measured by the quenching of chlorophyll fluorescence and is distinguished from photochemical quenching by applying a bright light pulse to transiently saturate photochemical quenching thus removing its contribution from the observed quenching. Non-photochemical quenching is not affected if the pulse of light is short. During the pulse, the fluorescence reaches the level reached in the absence of any photochemical quenching, known as maximum fluorescence, .

For further discussion, see Measuring chlorophyll fluorescence and Plant stress measurement.

Chlorophyll fluorescence can easily be measured with a chlorophyll fluorometer. Some fluorometers (such as the OS-5p) can calculate NPQ and photochemical quenching coefficients (including qP, qN, qE and NPQ), as well as light and dark adaptation parameters (including Fo, Fm, and Fv/Fm).

See also

Chlorophyll fluorescence

Measuring chlorophyll fluorescence

References

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         5.   Jörg Standfuss, Anke C Terwisscha van Scheltinga, Matteo Lamborghini and Werner Kühlbrandt, Mechanisms of photoprotection and nonphotochemical quenching in pea lightharvesting complex 
at 2.5A° resolution The EMBO Journal VOL 24 | NO 5 | 200
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    In its statement, the singapore property listing - website link, government claimed that the majority citizens buying their first residence won't be hurt by the new measures. Some concessions can even be prolonged to chose teams of consumers, similar to married couples with a minimum of one Singaporean partner who are purchasing their second property so long as they intend to promote their first residential property. Lower the LTV limit on housing loans granted by monetary establishments regulated by MAS from 70% to 60% for property purchasers who are individuals with a number of outstanding housing loans on the time of the brand new housing purchase. Singapore Property Measures - 30 August 2010 The most popular seek for the number of bedrooms in Singapore is 4, followed by 2 and three. Lush Acres EC @ Sengkang

    Discover out more about real estate funding in the area, together with info on international funding incentives and property possession. Many Singaporeans have been investing in property across the causeway in recent years, attracted by comparatively low prices. However, those who need to exit their investments quickly are likely to face significant challenges when trying to sell their property – and could finally be stuck with a property they can't sell. Career improvement programmes, in-house valuation, auctions and administrative help, venture advertising and marketing, skilled talks and traisning are continuously planned for the sales associates to help them obtain better outcomes for his or her shoppers while at Knight Frank Singapore. No change Present Rules

    Extending the tax exemption would help. The exemption, which may be as a lot as $2 million per family, covers individuals who negotiate a principal reduction on their existing mortgage, sell their house short (i.e., for lower than the excellent loans), or take part in a foreclosure course of. An extension of theexemption would seem like a common-sense means to assist stabilize the housing market, but the political turmoil around the fiscal-cliff negotiations means widespread sense could not win out. Home Minority Chief Nancy Pelosi (D-Calif.) believes that the mortgage relief provision will be on the table during the grand-cut price talks, in response to communications director Nadeam Elshami. Buying or promoting of blue mild bulbs is unlawful.

    A vendor's stamp duty has been launched on industrial property for the primary time, at rates ranging from 5 per cent to 15 per cent. The Authorities might be trying to reassure the market that they aren't in opposition to foreigners and PRs investing in Singapore's property market. They imposed these measures because of extenuating components available in the market." The sale of new dual-key EC models will even be restricted to multi-generational households only. The models have two separate entrances, permitting grandparents, for example, to dwell separately. The vendor's stamp obligation takes effect right this moment and applies to industrial property and plots which might be offered inside three years of the date of buy. JLL named Best Performing Property Brand for second year running

    The data offered is for normal info purposes only and isn't supposed to be personalised investment or monetary advice. Motley Fool Singapore contributor Stanley Lim would not personal shares in any corporations talked about. Singapore private home costs increased by 1.eight% within the fourth quarter of 2012, up from 0.6% within the earlier quarter. Resale prices of government-built HDB residences which are usually bought by Singaporeans, elevated by 2.5%, quarter on quarter, the quickest acquire in five quarters. And industrial property, prices are actually double the levels of three years ago. No withholding tax in the event you sell your property. All your local information regarding vital HDB policies, condominium launches, land growth, commercial property and more

    There are various methods to go about discovering the precise property. Some local newspapers (together with the Straits Instances ) have categorised property sections and many local property brokers have websites. Now there are some specifics to consider when buying a 'new launch' rental. Intended use of the unit Every sale begins with 10 p.c low cost for finish of season sale; changes to 20 % discount storewide; follows by additional reduction of fiftyand ends with last discount of 70 % or extra. Typically there is even a warehouse sale or transferring out sale with huge mark-down of costs for stock clearance. Deborah Regulation from Expat Realtor shares her property market update, plus prime rental residences and houses at the moment available to lease Esparina EC @ Sengkang